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淮山的组织培养与快速繁殖

以淮山(Dioscorea fordii Prain et Burkill)茎段为材料,以MS为基本培养基,在(25±1)℃、光照强度24~30μmol/m2.s-1、光照时间10 h/d条件下对淮山的组织培养与快速繁殖技术进行了研究.结果表明,组合MS+NAA0.03  (本文共6页) 阅读全文>>

《Chinese Herbal Medicines》2011年01期
Chinese Herbal Medicines

Simultaneous Determination of Four Major Steroidal Saponins in Seven Species of Dioscorea L.by HPLC-ELSD

Objective To control the quality of the species in Dioscorea L.better.Methods An HPLC-ELSD method was developed for the first time to simultaneously determine four bioactive ingredients:dioscin,gracillin,protoneodioscin,and protoneogracillin in 31 samples belonging to seven species of Dioscorea L.from different areas.The column was an Inertsil HILIC(250 mm×4.6 mm,5μm).The separation was carried out with a gradient pr...  (本文共5页) 阅读全文>>

《Medicinal Plant》2011年01期
Medicinal Plant

Study on Processing Technology of Preserved Fruit from Dioscorea alata Linn.

[Objective] To optimize the processing technology of preserved fruit from Dioscorea alata. [Method] Dioscorea alata from Hainan was taken as test material and treated with compound color-preserving reagents, then dried by microwave method, blowing method and microwave-blowing method respectively. [Result]The optimum process for the production of high qual...  (本文共3页) 阅读全文>>

《Medicinal Plant》2011年02期
Medicinal Plant

Sub-cloning and Expression of Dioscorea nipponica Glycosidase Gene

[Objective] To sub-clone and express glycosidase gene of Dioscorea nipponica. [Method]The glycosidase gene of Dioscorea nipponica was sub-cloned into Pichia pastoris GS115 expression vector pPIC9K, and the constructed expression vector w...  (本文共4页) 阅读全文>>

《Medicinal Plant》2010年11期
Medicinal Plant

Components Analysis of Amino Acids and Mineral Elements in Rhizoma Dioscorea cirrhosae

[Object] To analyze and determine the types and contents of amino acids and mineral elements in Rhizoma Dioscorea cirrhosae,so as to provide theoretical evidence for the development and utilization of Rhizoma Dioscorea cirrhosae.[Method] Contents of amino acids and mineral elements were determined by HPLC and inductively coupled plasma mass spectrometry respectively.[Result] 16 amino a...  (本文共3页) 阅读全文>>

《Medicinal Plant》2010年07期
Medicinal Plant

Effects of Different Growth-regulators on Shoot Regeneration Capacity of Dioscorea oppasita

[Objective]To provide basic data for establishing a virus-free system for stem tips of Dioscorea oppasita by studying the comprehensive effects of different growth-regulators with different concentration on the shoot regeneration capacity.[Method]With 6-BA,IBA,KT and CH as independent variables and differentiation rate,proliferation coefficient(total amount of reg...  (本文共3页) 阅读全文>>