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dUTP/UDG反应体系在荧光定量PCR中抗污染能力评价

目的 探讨dUTP/UDG反应体系在荧光定量PCR中抗PCR产物污染的能力。方法 用TaqMan荧光定量PCR检测方法进行定量检测。将一系列不同浓度的含dUTP的HBV DNA PCR扩增产物作为污染源 ,分别加至含dUTP/UDG的PCR反应体系和普通荧光定量PCR反应体系中 ,在荧光定量PCR仪上进行定量检测 ,从而得出含dUTP/UDG  (本文共4页) 阅读全文>>

《实用医技杂志》2007年01期
实用医技杂志

改进dUTP缺口末端标记技术法的操作体会

通过对多种石蜡组织切片运用dUTP缺口末端标记技术(TUNEL)法检测...  (本文共2页) 阅读全文>>

《Journal of Medical Colleges of PLA》2003年04期
Journal of Medical Colleges of PLA

Analysis of gene expression profile of aspermia using cDNA microarray

Objective: To identify the differential gene expression profiles between the normal and aspermia human testes utilizing cDNA microarray. Methods: cDNA probes were prepared by labeling mRNA of aspermia testes tissues with Cy5-dUTP and mRNA of normal testes tissues with Cy3-dUTP respectively through reverse transcription. The mixed cDNA probes were then hybridized with 4096 cDNA arrays (4096 unique human c...  (本文共5页) 阅读全文>>

《生命的化学》2002年04期
生命的化学

dUTP焦磷酸酶研究进展

dUTPase通过催化水解dUTP,降低尿嘧啶在DNA中的错误掺入,调节dUTP/dTTP的正常比例,保证了DNA复制的正确性和顺利进行。绝大多数dUTPase结构相似,活性中心由不同的亚基共同参与。dU...  (本文共4页) 阅读全文>>

《Asian Journal of Andrology》2004年01期
Asian Journal of Andrology

Expression and significance of Rap1A in testes ofazoospermic subjects

Aim: To evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects. Methods: A cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1 AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through...  (本文共6页) 阅读全文>>

《World Journal of Gastroenterology》2003年04期
World Journal of Gastroenterology

Analysis of gene expression profile of pancreatic carcinoma using cDNA microarray

AIM:To identify new diagnostic markers and drug targets,the gene expression profiles of pancreatic cancer werecompared with that of adjacent normal tissues utilizing cDNAmicroarray analysis.METHODS:cDNA probes were prepared by labeling mRNAfrom samples of six pancreatic carcinoma tissues with Cy5-dUTP and mRNA from adjacent normal tissues with Cy3-dUTP respectively through reverse transcription.The mixedprobes of eac...  (本文共6页) 阅读全文>>