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神经干细胞体外增殖与分化的部分影响因子探讨

目的 探讨神经营养因子bFGF、EGF和N2添加剂对体外培养的神经干细胞增殖的影响;研究bFGF、BMP-2、ATRA、VD3、DEX对神经干细胞分化的诱导作用及该作用与维甲酸受体mRNA表达的关系。方法 应用细胞计数和免疫荧光细胞化学法,研究不同神经营养因子对神经干细胞增殖及分化的影响;应用RT-PCR、琼脂糖凝胶电泳和紫外分光图象分析法检测神经干细胞分化过程中RARα和RXRαmRNA表达量的改变。结果1.在bFGF、EGF和N2组成的八种不同培养液中,bFGF和b*E*N(bFGF+EGF+N2的简称,余类推)两组是最佳选择配方;bFGF对神经干细胞的生长是必不可少的;EGF主要刺激细胞分化;N2则导致大量单细胞的生成,这些单细胞既不形成细胞团也不贴壁分化,最终退化死亡。2.细胞分化中如果缺少bFGF,则分化细胞快速死亡;BMP-2、ATRA和R*V*D(ATRA+VD3+DEX的简称,余类推)对神经干细胞分化具明显诱  (本文共52页) 本文目录 | 阅读全文>>

《中成药》2014年05期
中成药

五子衍宗丸对ATRA诱导的神经管畸形防治作用初探

目的初步研究五子衍宗丸对全反式维甲酸(all-trans-retinoic acid,ATRA)诱导的神经管畸形(neural tube defects,NTDs)的防治作用,并筛选出五子衍宗丸防治NTDs的最佳给药方式。方法 BALB/c雄鼠和雌鼠以1∶2比例合笼,有阴栓者为孕鼠。将110只孕鼠随机分为正常组、模型组、五子衍宗丸先给组、五子衍宗丸和ATRA同时干预组、五子衍宗丸后给组,后3组又各分为五子衍宗丸高(4.20 g/kg...  (本文共3页) 阅读全文>>

《中国现代医生》2011年20期
中国现代医生

ATRA联合三氧化二砷在急性早幼粒细胞白血病治疗中的临床应用研究

目的探讨全反式维甲酸(all-trans retinoic acid,ATRA)联合三氧化二砷治疗急性早幼粒细胞白血病的临床疗效。方法选取24例经临床诊断、血象、骨髓象、细胞免疫、细胞遗传学等检查确诊为急性...  (本文共2页) 阅读全文>>

《Cellular & Molecular Immunology》2007年01期
Cellular & Molecular Immunology

Down-Regulation of CD44 Contributes to the Differentiation of HL-60 Cells Induced by ATRA or HMBA

CD44 is highly expressed in human acute myeloid leukemia (AML) cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid (ATRA) an...  (本文共5页) 阅读全文>>

《Journal of Medical Colleges of PLA》2006年04期
Journal of Medical Colleges of PLA

Effects of varied interferons in combination with all-trans retinoic acid ( ATRA ) on proliferation and differentiation of ATRA-resistent APL cell

Objective: To investigate the effects and mechanisms of interferon in combination with all-trans retinoic acid (ATRA) on proliferation and differentiation of ATRA-resistent APL cell. Methods:After MR2 cells (ATRA-resistance cell line) were treated with IFN-α, IFN-γand ATRA alone or IFN-αand IFN-γin combination with ATRA respectively. The cell proliferation was tested by MTT test and the cell differentiation was teste...  (本文共6页) 阅读全文>>

《华中科技大学学报(医学英德文版)》2004年04期
华中科技大学学报(医学英德文版)

The Effect of Sodium Butyrate in Combination with ATRA on the Proliferation/Differentiation of SKM-1

To explore the molecular mechanisms of sodium butyrate working on SKM-1 cell proliferation/differentiation and to study its synergistic effect with all-trans retinoic acid (ATRA), SKM-1 cells were grown in the absence or presence of sodium butyrate and/or ATRA. The percentage of viable cells was determined by trypan blue exclusion. Differentiation was determined by nitroblue tetrazolium (NBT) reduction and cell surfa...  (本文共4页) 阅读全文>>