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携带TIMP-4 cDNA的重组腺病毒载体的构建

目的克隆细胞外基质金属蛋白酶组织抑制因子4(tissue inhibitor-4 of metalloproteinases,TIMP-4)基因,构建包装携带TIMP-4基因的重组腺病毒载体(Ad/T4),为基因治疗血管再狭窄的实验研究打下基础。方法以人心脏cDNA文库为模板,应用PCR克隆TIMP-4基因,经  (本文共6页) 阅读全文>>

《郑州大学学报(医学版)》2006年03期
郑州大学学报(医学版)

TIMP-1绿色荧光蛋白真核表达载体的构建和鉴定

目的:构建并鉴定TIMP1绿色荧光蛋白真核表达载体。方法:提取胃癌组织总RNA,用TIMP1基因引物进行RT- PCR,将扩增产物克隆至pGEM -T载体,PCR鉴定及测序证实后,BamHⅠ和HindⅢ双酶切pGEM- T- TIMP1重组体,回收TIMP1,再将...  (本文共3页) 阅读全文>>

《华中科技大学学报(医学英德文版)》2006年03期
华中科技大学学报(医学英德文版)

Modulation of Matrix Metalloproteinase and TIMP-1 Expression by TGF-β_1 in Cultured Human RPE Cells

In order to investigate the effects of TGF-β_ 1 on the expression of MMP-2, -9 and TIMP-1 in human retinal pigment epithelial (RPE) cells, the third-sixth passage cultured RPE cells were treated with TGF-β_ 1 at different concentrations (0.01, 0.1, 1.0, 10 ng/mL), the expression of MMP-2, -9 and TIMP-1 mRNA was detected by semi-quantitative RT-PCR assays. MMP-2,...  (本文共3页) 阅读全文>>

《World Journal of Gastroenterology》2006年19期
World Journal of Gastroenterology

Correlation between TIMP-1 expression and liver fibrosis in two rat liver fibrosis models

AIM: To evaluate serum TIMP-1 level and the correlation between TIMP-1 expression and liver fibrosis in immune-induced and CCL4-induced liver fibrosis models in rats. METHODS: Immune-induced and CCL4-induced liver fibrosis models were established by dexamethasone (0.01 mg) and CCL4 respectively. Serum TIMP-1 level was detected with ELISA, while histopathological grade of liver biopsy was evaluated. Spearman rank-corr...  (本文共6页) 阅读全文>>

《Cell Research》2006年09期
Cell Research

Coordinated peak expression of MMP-26 and TIMP-4 in preinvasive human prostate tumor

The identification of novel biomarkers for early prostate cancer diagnosis is highly important because early detectionand treatment are critical for the medical management of patients.Disruption in the continuity of both the basal celllayer and basement membrane is essential for the progression of high-grade prostatic intraepithelial neoplasia (HGPIN)to invasive adenocarcinoma in human prostate.The molecules involved...  (本文共9页) 阅读全文>>

《皖南医学院学报》2006年01期
皖南医学院学报

子宫内膜异位症中TIMP-1基因表达的研究

目的研究TIMP-1基因在子宫内膜异位症发生中的作用。方法采用免疫组织化学SP法研究25例内异症和22例正常子宫内膜组织中TIMP-1的蛋白表达情况;采用RT-PCR方法研究33例内异症的异位子宫内膜组织和3...  (本文共4页) 阅读全文>>