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基因工程菌株产生含人类绒毛膜促性腺激素抗原的研究 Ⅱ.β—galactosidase—hCG聚体蛋白免疫原性

基因工程菌株产生的含人类绒毛膜促性腺激素(hCG)β亚单位C末端36肽的β-半乳糖苷酶杂交蛋白(简称β-galaclosidase-hCG),在未经解聚处理前免疫新西兰种白兔和昆明种小白鼠,其抗血清在免疫双扩散试验和对流免疫电泳中对  (本文共7页) 阅读全文>>

《Chinese Medical Journal》2007年13期
Chinese Medical Journal

B to O erythrocyte conversion by the recombinant α-galactosidase

Background Human group O red blood cells have great benefit in specialized transfusion areas such as armed conflictand natural calamity.The group B antigen differs structurally from group O antigen only by the addition of one terminalα-linked galactose residue.In this study we aimed to remove the terminal galactose from group B red blood cell to getgroup O red blood cell.Methods α-galactosidase cDNA was cloned by RT-...  (本文共6页) 阅读全文>>

《华中科技大学学报(医学英德文版)》2000年04期
华中科技大学学报(医学英德文版)

Experiments on Gene Transferring to Primary Hematopoietic Cells by Liposome

Summary Liposomes have showed many advantages in mediating exogenous gene into many cell types in vitro and in vivo. But few data are available concerning gene transfer into hematopoietic cells. In this report, we described two-marker genes (Neo R and Lac Z) co-transferred into hematopoietic cells of human and mouse by using liposome in vitro. The efficiency of gene transfer was tested by X- gal staining and obser...  (本文共3页) 阅读全文>>

《Journal of Microbiology and Immunology》2004年04期
Journal of Microbiology and Immunology

Construction and Expression of β-galactosidase Genetically Engineered Lactococcus lactis

Our objective is to solve the lactose malabsorption and intolerance of human beings by combining micro-ecology path with genetic engineering technique. Plasmid pMG36e was used to clone and express a β-galactosidase gene from L. delbrueckü bulgaricus strain 1.1480 in the Lactococcus lactis subsp. cremoris MG1363 and Lactococcus lactis subsp. lactis IL1403. The recombinant plasmid was preserved and proliferated in Esch...  (本文共7页) 阅读全文>>

《Rice Science》2007年04期
Rice Science

Changes in Activities of Three Enzymes Degrading Galactomannan During and Following Rice Seed Germination

To investigate the relationships among β-mannanase, β-mannosidase and α-galactosidase required for degrading galactomannan in cell wall during and following rice seed germination, the activities of the three enzymes and the effects of ABA and GA3 on them were surveyed. The activiti...  (本文共7页) 阅读全文>>

《Biomedical and Environmental Sciences》2008年05期
Biomedical and Environmental Sciences

Non-Fusion and Fusion Expression of β-Galactosidase from Lactobacillus bulgaricus in Lactococcus lactis

Objective To construct four recombinant Lactococcus lactis strains exhibiting high β-galactosidase activity in fusion or non-fusion ways,and to study the influence factors for their protein expression and secretion.Methods The gene fragments encoding β-galactosidase from two strains of Lactobacillus bulgaricus,wch9901 isolated from yogurt and 1.1480 purchased from the Chinese Academy of Sciences,were amplified and in...  (本文共9页) 阅读全文>>