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粉防己碱抑制肝星状细胞活化与转化生长因子-β信号转导关系

目的观察不同剂量粉防己碱对静止期大鼠肝星状细胞(HSC)活化的影响,以及该作用与转化生长因子-β(TGF-β)信号转导的关系。方法采用胶原酶原位灌注、密度梯度离心法分离正常大鼠HSC,培养48h后并给予不同浓度粉防己碱(0.25、0.5、1.0、2.0mg/L)处理。观察HSC形态学变化。采用免疫细胞化学法检测α-平滑肌肌动蛋白(α-SMA)表达并进行图像分析;放射免疫法检测上清层粘连蛋白(LN)和Ⅲ型前胶原(PCⅢ);逆转录聚合酶链反应(RT-PCR)检测  (本文共4页) 阅读全文>>

《华中科技大学学报(医学英德文版)》2005年06期
华中科技大学学报(医学英德文版)

Wild-type Smad3 Gene Enhances the Osteoblastic Differentiation of Rat Bone Marrow-derived Mesenchymal Stem Cells in Vitro

This study examined the effect of wild-type Smad3 gene on the osteoblastic differentiation of rat bone marrow-derived mesenchymal stem cells in vitro. Bone marrow-derived mesenchymal stem cells (MSCs) were stably transfected with the complexes of pcDNA3.0-Myc-Smad3 or pcDNA3.0-Myc-Smad3△C and Lipofectamine reagent. Immunofluorescence staining was performed to evaluate the c-Myc signal in MSCs. The cell proliferation ...  (本文共5页) 阅读全文>>

《华中科技大学学报(医学英德文版)》2005年01期
华中科技大学学报(医学英德文版)

Influence of Exogenous TGFβ_1 on the Expression of Smad2 and Smad3 in Rat Bone Marrow-derived Mesenchymal Stem Cells

The expression of Smad2 and Smad3 and the influence of exogenous transforming growth factorβ 1 (TGFβ 1) on them in rat bone marrow-derived mesenchymal stem cells (MSCs) cultured in vitro were investigated. The effects of different concentrations of TGFβ 1 on cell proliferation and ALP activity were detected by MTT and PNPP in MSCs respectively. The expression of Smad2 and Smad3 and the influence of exogenous...  (本文共4页) 阅读全文>>

《国外医学.流行病学传染病学分册》2005年04期
国外医学.流行病学传染病学分册

Smad蛋白与肝纤维化

肝星形细胞(HSC)是肝纤维化发生发展的关键细胞,转化生长因子β1(TGF-β1)是促进HSC活化、细胞外基质(EC...  (本文共4页) 阅读全文>>

《Medicinal Plant》2020年01期
Medicinal Plant

Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease

[Objectives] This study aimed to study the effects of Shenge Yifei capsule on the TGF-β1/Smad signaling pathway in rats with chronic obstructive pulmonary disease(COPD). [Methods] Ten rats were randomly selected as the control group, and the other 40 rats were selected for modeling by fumigation combined with Klebsiella pneumoniae infection. A total of 38 rats were successfully modeled. They were randomly divided int...  (本文共4页) 阅读全文>>

《Chinese Journal of Integrative Medicine》2020年05期
Chinese Journal of Integrative Medicine

Linggui Zhugan Decoction(苓桂术甘汤) Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Rats by Suppressing TGF-β1/Smad Signaling Pathway

Objective: To investigate the inhibitory effect of Linggui Zhugan Decoction(LZD, 苓桂术甘汤) on the ventricular remodeling(VR) after acute myocardial infarction(AMI) and related m RNA and proteins expression in transforming growth factor-beta 1(TGF-β_1)/Smad signaling pathway, and explain its putative mechanism. Methods: A VR model was generated by ligation of coronary artery in rats. Two weeks after surgery, 60 rats were...  (本文共8页) 阅读全文>>