TGFβ/Smad信号通路在口腔鳞癌热疗中的表达变化

目的:探讨TGFβ/Smad信号通路在口腔鳞状细胞癌(OSCC)热疗之后相关分子的表达变化。方法:利用实时荧光定量RT-PCR和Western blot检测局部加热对OSCC荷瘤裸鼠肿瘤细胞中TGFβ,Smad2,Smad3,Smad4和Smad7的mRNA和蛋白表达的变化。结果:加热组(n=10)中TGFβ、Smad2、S  (本文共4页) 阅读全文>>

Journal of Traditional Chinese Medicine

Wulong Xiaozheng Wan medicated serum inhibits epithelial-mesenchymal transition in human gastric carcinoma cell line BGC823 by modulation of transforming growth factor-β_1/Smad signaling

OBJECTIVE:To evaluate the effect of Wulong Xiaozheng Wan medicated serum on the epithelial-mesenchymal transition(EMT)of BGC823 cell induced by transforming growth factor-β_1(TGF-β_1)and to explore its mechanism.METHODS:EMT model of BGC823 was stimulated by TGF-β_1.Wulong Xiaozheng Wan medicated serum and LY-364947 were used as intervention.The proliferation and adhesion of BGC823 were detected by 3-(4,5-dimethyl-2-t...  (本文共13页) 阅读全文>>

Medicinal Plant

Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease

[Objectives] This study aimed to study the effects of Shenge Yifei capsule on the TGF-β1/Smad signaling pathway in rats with chronic obstructive pulmonary disease(COPD). [Methods] Ten rats were randomly selected as the control group, and the other 40 rats were selected for modeling by fumigation combined with Klebsiella pneumoniae infection. A total of 38 rats were successfully modeled. They were randomly divided int...  (本文共4页) 阅读全文>>

Chinese Journal of Integrative Medicine

Linggui Zhugan Decoction(苓桂术甘汤) Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Rats by Suppressing TGF-β1/Smad Signaling Pathway

Objective: To investigate the inhibitory effect of Linggui Zhugan Decoction(LZD, 苓桂术甘汤) on the ventricular remodeling(VR) after acute myocardial infarction(AMI) and related m RNA and proteins expression in transforming growth factor-beta 1(TGF-β_1)/Smad signaling pathway, and explain its putative mechanism. Methods: A VR model was generated by ligation of coronary artery in rats. Two weeks after surgery, 60 rats were...  (本文共8页) 阅读全文>>

International Journal of Ophthalmology

Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2,3 signal transduction in vitro

AIM:To research the effect of Y-27632,a selective Rhoassociated coiled-coil kinase(ROCK) inhibitor,on TGF-β1/Smad2,3 signal transduction in ocular Tenon's capsule fi broblasts(OTFs).METHODS:Primary ocular Tenon's capsule fibroblasts had been cultured in vitro.The effect of Y27632 on proliferation of OTF stimulated by lysophosphatidic acid(LPA) was evaluated by MTT colorimetric assay so as to sift out the proper conce...  (本文共7页) 阅读全文>>

健康之路

脑星形细胞瘤组织TGF-β1、Smad7与VEGF的表达及意义

目的:探讨TGF-β1、Smad7与VEGF在星形细胞瘤组织中的表达及意义。方法:选择我院收治的35例脑星形细胞瘤患者以及15例脑外伤内减压术正常脑组织患者作为实验研究对象。对正常脑组织与脑星形细胞瘤组织均采取免疫组化法进行检测,探讨不同级别星形细胞瘤组织中...  (本文共1页) 阅读全文>>