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甲酸脱氢酶在Klebsiella pneumoniae中的表达和功能分析

在甘油厌氧发酵生产1,3-丙二醇的过程中,需要消耗还原当量NADH,NADH的有效供给决定了1,3-丙二醇的产量和得率。采用PCR方法从Candidaboidinii基因组中克隆编码甲酸脱氢酶基因fdh,将fdh基因片段插入载体pMALTM-p2X中,构建表达载体pMALTM-p2X-fdh,并转入1,3-丙二醇生产菌Kleb  (本文共5页) 阅读全文>>

《Chinese Science Bulletin》2004年16期
Chinese Science Bulletin

Construction and characteri-zation of double mutants in nitrogenase of Klebsiella pneumoniae

Two mutants in nitrogenase of Klebsiella pneu-moniae are constructed by site-directed mutagenesis and gene replacement procedure, which express the nitrogenases with Lysine and Glutamine substituting for a-Glutamine 190 and a-Histidine 194 respectively (Kp-Q a190 K and Kp-H a194 Q). The above two substitutions are respectively intro-duced into a nifV mutant (expressing a citrate-containing nitrogenase) and sequential...  (本文共7页) 阅读全文>>

《Chinese Medical Journal》1995年05期
Chinese Medical Journal

Bacterial Resistance in Clinical Isolates of Klebsiella Pneumoniae

Ninety-four percent of 336 strains Klebsiellapneumoniae were resistant to ampicillin,while...  (本文共1页) 阅读全文>>

《Science Foundation in China》2019年04期
Science Foundation in China

Fatty liver disease caused by high alcohol-producing Klebsiella pneumoniae

With the support by the National Natural Science Foundation of China,the research team led by Prof. Yuan Jing(袁静) from the Capital Institute of Pediatrics,Liu Di (刘翟) from Wuhan Institute of Virology,Chinese Academy of...  (本文共1页) 阅读全文>>

《China Medical Abstracts(Internal Medicine)》2019年04期
China Medical Abstracts(Internal Medicine)

Resistance genotype,capsular serotype and virulence gene of hypermucoviscous Klebsiella pneumoniae in Anhui Province

Objective To investigate the resistance genotype,capsular serotype and virulence g...  (本文共1页) 阅读全文>>

《福建农林大学学报(自然科学版)》2020年01期
福建农林大学学报(自然科学版)

氮对巨菌草内生固氮菌Klebsiella variicola GN02 nifH基因表达的影响

采用DNA电泳、qRT-PCR及Western blot技术分析了在无氮和含氮培养条件下,巨菌草内生固氮菌Klebsiella variicola GN02菌株nifH基因序列长度、基因与蛋白表达...  (本文共6页) 阅读全文>>